Wykorzystanie Ekstrakcji Sekwencyjnej Do Oceny Zagrożeń Dla Środowiska Powodowanych Przez Żużle I Popioły Z Procesu Termicznego Przekształcania Odpadów

Thermal treatment of waste is one of the ways of their processing. It is commonly used in most developed countries of the European Union. Major by-products of the combustion processes are slag and bottom ash. In the majority of EU countries bottom ash and slag are used as a priming for road construction. In Poland slag and bottom ash from incineration process are stabilized with the addition of cement and some polymers and are landfilled as wastes. In accordance to Polish law, depending on the leaching of heavy metals from fly ash and slag after thermal treatment of waste can be regarded as both hazardous and non-hazardous wastes. At present work sequential extraction methods described in the literature: Tessier’s method, van Herck’s method and BCR method were compared experimentally with the results of using Swiss standard TVA.SA.1991 and European standard EN 12457 and total concentration of metals in sample analyzed after complete digestion of sample. The study sample was bottom ash from the medical waste incineration plant.Termiczne przekształcanie odpadów jest jednym ze sposobów ich przetwarzania. Głównymi produktami ubocznymi procesu spalania są żużel i popiół paleniskowy. W większości krajów UE są one wykorzystywane gospodarczo. W Polsce żużle i popioły po termicznym przekształcaniu stabilizuje się z dodatkiem cementu i polimerów i składuje jako odpad. Zgodnie z obowiązującym prawem, w zależności od wymywalności metali ciężkich, żużel i popioły po termicznym przekształcaniu odpadów mogą być uznawane zarówno za odpady niebezpieczne, jak i inne niż niebezpieczne. W pracy porównano doświadczalnie metody ekstrakcji sekwencyjnej opisane w literaturze: metodę Tessiera, metodę van Hercka oraz metodę BRC. Badaniu poddano próbkę popiołu pochodzącą ze spalarni odpadów medycznych

Analysis of Linoprint distribution channels, development of marketing concepts and tools

PFC del programa Erasmus EPS elaborat a Fachhochschule Kiel University of Applied SciencesTreball desenvolupat dins el marc del programa 'European Project Semester'.The objective of this report is to analyze the effectiveness of current Linoprint distributors and potential new distributors, to analyze Linoprint’s company strengths compared to its competitors in the digital printing market, to develop Microsoft Excel based marketing tools, and to propose marketing concepts to Linoprint by analyzing Linoprint’s current marketing material and the package printing industry, which is the market that Linoprint is active in. The results of the project are used to develop tools and recommendations to improve Linoprint’s marketing and current company positioning, and to recommend strategies for Linoprint to consider as it enters the market

Can we further improve the quality of nephro-urological care in children with myelomeningocele?

Myelomeningocele (MMC) results from a failure of normal neural tube fusion in early fetal development. Retrospective, observational study of medical data of 54 children treated in Pediatric Nephrology and Urology Clinics for five years was performed. The following data were analyzed: serum creatinine, eGFR, urine analysis, renal scintigraphy (RS), renal ultrasound, and urodynamics. Mean age of studied population: 12.3 years, median of eGFR at the beginning and at the end of survey was 110.25 and 116.5 mL/min/1.73 m2 accordingly. Median of frequency of urinary tract infections (fUTI): 1.2 episodes/year. In 24 children: low-pressure, in 30 children: high-pressure bladder was noted. Vesicouretral reflux (VUR) was noted in 23 children (42.6%). fUTI were more common in high-grade VUR group. High-grade VURs were more common in group of patients with severe renal damage. At the end of the survey 11.1% children were qualified to higher stages of chronic kidney disease. Renal parenchyma damage progression in RS was noted in 22.2% children. Positive VUR history, febrile recurrent UTIs, bladder wall trabeculation, and older age of the patients constitute risk factors of abnormal renal scans. More than 2.0 febrile, symptomatic UTIs annually increase by 5.6-fold the risk of severe renal parenchyma damage after five years

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome

Background The DiGeorge syndrome, the most common of the microdeletion syndromes, affects multiple organs, including the heart, the nervous system, and the kidney. It is caused by deletions on chromosome 22q11.2; the genetic driver of the kidney defects is unknown. Methods We conducted a genomewide search for structural variants in two cohorts: 2080 patients with congenital kidney and urinary tract anomalies and 22,094 controls. We performed exome and targeted resequencing in samples obtained from 586 additional patients with congenital kidney anomalies. We also carried out functional studies using zebrafish and mice. Results We identified heterozygous deletions of 22q11.2 in 1.1% of the patients with congenital kidney anomalies and in 0.01% of population controls (odds ratio, 81.5; P=4.5×10(-14)). We localized the main drivers of renal disease in the DiGeorge syndrome to a 370-kb region containing nine genes. In zebrafish embryos, an induced loss of function in snap29, aifm3, and crkl resulted in renal defects; the loss of crkl alone was sufficient to induce defects. Five of 586 patients with congenital urinary anomalies had newly identified, heterozygous protein-altering variants, including a premature termination codon, in CRKL. The inactivation of Crkl in the mouse model induced developmental defects similar to those observed in patients with congenital urinary anomalies. Conclusions We identified a recurrent 370-kb deletion at the 22q11.2 locus as a driver of kidney defects in the DiGeorge syndrome and in sporadic congenital kidney and urinary tract anomalies. Of the nine genes at this locus, SNAP29, AIFM3, and CRKL appear to be critical to the phenotype, with haploinsufficiency of CRKL emerging as the main genetic driver. (Funded by the National Institutes of Health and others.)

Ocena zagrożenia środowiska wynikająca z zagospodarowania popiołów z termicznego przekształcania odpadów

Proces termicznego przekształcania odpadów staje się coraz bardziej atrakcyjnym i pożądanym rozwiązaniem w gospodarce odpadami. Termiczne przekształcanie odpadów jest to proces spalania, który prowadzi do zredukowania ilości odpadów składowanych na składowiskach, przy jednoczesnym wytworzeniu energii elektrycznej i cieplnej [1-3]

Effect of Various Cooking Methods on Technological and Sensory Quality of Atlantic Salmon (Salmo salar)

The aim of the study was to evaluate the effect of heat treatment salmon quality using the sous-vide method (57 °C, 20 min and 63 °C, 80 min) in comparison with traditional methods (steam cooking, roasting). The yield of process and sensory quality (sensory profile, consumer liking) and the color and texture of salmon was measured. Salmon processed with the sous-vide method was characterized by a statistically significantly (p ≤ 0.05) higher yield and water content than the samples prepared by steaming and roasting. Statistically higher (p ≤ 0.05) consumer preference for salmon prepared using higher parameters of the sous-vide process (63 °C, 80 min) compared with low parameters (57 °C, 20 min) was stated. Parameters of the sous-vide processing (57 °C, 20 min) have a negative effect on salmon quality. It was observed that as the temperature and time of the process increased, the intensity of the flavor and odor attributes of cooked fish also increased, while the intensity of raw fish, juiciness, tenderness, and softness decreased. The color of salmon also changed. Based on the results, we recommend the following parameters of process in preparing salmon using the sous-vide method: 63 °C for 80 min

Identification of a ferritin-like protein of <it>Listeria monocytogenes</it> as a mediator of β-lactam tolerance and innate resistance to cephalosporins

Abstract Background The food-borne pathogen Listeria monocytogenes is the causative agent of listeriosis. The β-lactam antibiotics penicillin G and ampicillin are the current drugs of choice for the treatment of listerial infections. While isolates of L. monocytogenes are susceptible to these antibiotics, their action is only bacteriostatic and consequently, this bacterium is regarded as tolerant to β-lactams. In addition, L. monocytogenes has a high level of innate resistance to the cephalosporin family of β-lactams frequently used to treat sepsis of unknown etiology. Given the high mortality rate of listeriosis despite rational antibiotic therapy, it is important to identify genes that play a role in the susceptibility and tolerance of L. monocytogenes to β-lactams. Results The hly-based promoter trap system was applied to identify penicillin G-inducible genes of L. monocytogenes. The results of reporter system studies, verified by transcriptional analysis, identified ten penicillin G-inducible genes. The contribution of three of these genes, encoding a ferritin-like protein (fri), a two-component phosphate-response regulator (phoP) and an AraC/XylS family transcriptional regulator (axyR), to the susceptibility and tolerance of L. monocytogenes to β-lactams was examined by analysis of nonpolar deletion mutants. The absence of PhoP or AxyR resulted in more rapid growth of the strains in the presence of sublethal concentration of β-lactams, but had no effect on the MIC values or the ability to survive a lethal dose of these antibiotics. However, the Δfri strain showed impaired growth in the presence of sublethal concentrations of penicillin G and ampicillin and a significantly reduced ability to survive lethal concentrations of these β-lactams. A lack of Fri also caused a 2-fold increase in the sensitivity of L. monocytogenes to cefalotin and cephradine. Conclusions The present study has identified Fri as an important mediator of β-lactam tolerance and innate resistance to cephalosporins in L. monocytogenes. PhoP and AxyR are probably involved in transmitting signals to adjust the rate of growth of L. monocytogenes under β-lactam pressure, but these regulators do not play a significant role in susceptibility and tolerance to this class of antibiotics.</p

Analysis of Linoprint distribution channels, development of marketing concepts and tools

PFC del programa Erasmus EPS elaborat a Fachhochschule Kiel University of Applied SciencesTreball desenvolupat dins el marc del programa 'European Project Semester'.The objective of this report is to analyze the effectiveness of current Linoprint distributors and potential new distributors, to analyze Linoprint’s company strengths compared to its competitors in the digital printing market, to develop Microsoft Excel based marketing tools, and to propose marketing concepts to Linoprint by analyzing Linoprint’s current marketing material and the package printing industry, which is the market that Linoprint is active in. The results of the project are used to develop tools and recommendations to improve Linoprint’s marketing and current company positioning, and to recommend strategies for Linoprint to consider as it enters the market

Analysis of Linoprint distribution channels, development of marketing concepts and tools

PFC del programa Erasmus EPS elaborat a Fachhochschule Kiel University of Applied SciencesTreball desenvolupat dins el marc del programa 'European Project Semester'.The objective of this report is to analyze the effectiveness of current Linoprint distributors and potential new distributors, to analyze Linoprint’s company strengths compared to its competitors in the digital printing market, to develop Microsoft Excel based marketing tools, and to propose marketing concepts to Linoprint by analyzing Linoprint’s current marketing material and the package printing industry, which is the market that Linoprint is active in. The results of the project are used to develop tools and recommendations to improve Linoprint’s marketing and current company positioning, and to recommend strategies for Linoprint to consider as it enters the market